Description

LL-37 (Cathelicidin)

LL-37 is the only member of the Cathelicidin family of antimicrobial peptides found in humans. Composed of 37 amino acids, this amphipathic alpha-helical peptide is released from the C-terminus of the hCAP18 protein by proteinase 3. In the field of advanced immunology and microbiology research, LL-37 is highly distinguished for its pleiotropic roles—extending far beyond simple pathogen neutralization. It acts as a critical immunomodulator, stimulating the chemotaxis of neutrophils, monocytes, and T-cells, and modulating the inflammatory response by neutralizing lipopolysaccharides (LPS). It provides researchers with a high-precision tool to study angiogenesis, the acceleration of re-epithelialization in wound healing, and the disruption of bacterial biofilms in persistent infection models.

Our LL-37 is synthesized in an ISO 9001:2015 certified and GMP-grade manufacturing environment. Utilizing advanced Solid-Phase Peptide Synthesis (SPPS) and high-resolution multi-stage HPLC purification, we achieve an ultra-high chemical purity of ≥99%. This meticulous production standard ensures the peptide is free from truncated sequences and residual synthesis reagents, guaranteeing the structural fidelity and biological activity required for sensitive research into host-defense mechanisms and cellular signaling.

---

Research Applications & Mechanisms

Antimicrobial & Biofilm Disruption

Investigating the peptide’s ability to permeate bacterial membranes and inhibit biofilm formation in various Gram-positive and Gram-negative experimental models.

Immunomodulation & Chemotaxis

Researching the pathways through which LL-37 recruits immune cells and interacts with Formyl Peptide Receptor 2 (FPR2) to study the regulation of the inflammatory cascade.

Wound Healing & Angiogenesis

Studying the stimulation of vascular endothelial growth factor (VEGF) and the promotion of keratinocyte migration to investigate accelerated tissue repair mechanisms.

LPS Neutralization

Exploring the capacity of LL-37 to bind and neutralize bacterial endotoxins, providing a model to study the mitigation of sepsis and systemic inflammatory responses.

---

Technical Specifications

Parameter	Laboratory Standard
Compound	LL-37 (Human Cathelicidin)
Sequence	Leu-Leu-Gly-Asp-Phe-Phe-Arg-Lys-Ser-Lys-Glu-Lys-Ile-Gly-Lys-Glu-Phe-Lys-Arg-Ile-Val-Gln-Arg-Ile-Lys-Asp-Phe-Leu-Arg-Asn-Leu-Val-Pro-Arg-Thr-Glu-Ser
Purity (HPLC)	≥99% (Pharmaceutical Grade)
Physical Appearance	Fine, Smooth & Flat Lyophilized Cake
Molecular Weight	4493.3 Da
Concentration	5mg per vial
Solubility	Soluble in Water / PBS / Bacteriostatic Water
Batch Control	MS & HPLC Analysis Verified

---

Key Features & Quality Assurance

Pharmaceutical-Grade Lyophilization

Our proprietary vacuum-sealing process ensures the LL-37 powder surface is perfectly smooth, flat, and delicate. This uniform “cake” structure ensures rapid, clear reconstitution and maintains the molecular stability of the complex 37-amino acid sequence during storage.

High-Fidelity Sequence Integrity

Every batch undergoes strict Mass Spectrometry (MS) to confirm the exact amino acid sequence, ensuring maximal receptor binding affinity and reproducible results in complex cellular assays.

---

Reconstitution & Storage

Reconstitution Guide

• Preparation: Allow the vial to reach room temperature. Centrifuge for 30-60 seconds to ensure the LL-37 cake is at the base.

• Solvent: Reconstitute with Sterile Water or Bacteriostatic Water.

• Technique: Gently introduce the diluent against the vial wall. DO NOT SHAKE; swirl gently until the solution is clear and transparent.

Storage Protocols

• Long-term: Store at -20°C (Maintains potency for up to 24 months).

• Short-term: Stable at 2–8°C for up to 3 months.

• Post-Reconstitution: Store at 2–8°C and utilize within 10-14 days for optimal results.

---

Shipping & Documentation

• Global Logistics: Shipped in discreet, temperature-controlled packaging to maintain the structural integrity of the LL-37 peptide.

• Verified Quality: A batch-specific COA and HPLC/MS reports are available for every order.

• Reshipment Policy: We provide a guaranteed reshipment policy for any lost or customs-intercepted orders (terms apply).

---

⚠️ Research Disclaimer

Product-Specific Safety Notice: This LL-37 is strictly for laboratory research and analytical purposes. It is NOT intended for human or veterinary administration. All research must be conducted by qualified professionals.

---

Scientific Resources & References

• National Center for Biotechnology Information (NCBI): LL-37 Peptide Summary & Antimicrobial Research

• ScienceDirect (Elsevier): L-37 antimicrobial peptide: Molecular characterisation and its role in oral health and disease–A narrative reviewP

---

🔍 Deep Dive Research

• [Technical Guide]: How to Reconstitute and Store Lyophilized Peptides for Maximum Potency

• [Immunity Study]: The Innate Frontier: Exploring LL-37 in Modern Infection and Healing Research