Description

PE-22-28 Peptide

PE-22-28 is a potent synthetic heptapeptide (Gly-Val-Trp-Arg-Asn-Gly-Arg) derived from the naturally occurring peptide Spadin. In the field of advanced neuropsychiatry and cellular biology research, PE-22-28 is highly distinguished as a specific blocker of the TREK-1 (K2P2.1) potassium channel. Unlike traditional monoamine-based research compounds, PE-22-28 exerts its effects by antagonizing TREK-1 channels in the hippocampus, leading to a rapid upregulation of Brain-Derived Neurotrophic Factor (BDNF) and the acceleration of neurogenesis. This mechanism provides researchers with a high-precision tool to study “fast-acting” antidepressant pathways and the molecular dynamics of neuronal plasticity within remarkably short observation windows.

Our PE-22-28 is synthesized in an ISO 9001:2015 certified and GMP-grade manufacturing environment. Utilizing state-of-the-art solid-phase synthesis and multi-stage HPLC purification, we achieve an ultra-high chemical purity of ≥99%. This meticulous production standard ensures the peptide is free from truncated isomers and residual synthesis reagents, guaranteeing the consistency required for sensitive behavioral modeling and electrophysiological assays.

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Research Applications & Mechanisms

TREK-1 Channel Antagonism

Investigating the selective blocking of two-pore domain potassium channels to study the modulation of neuronal excitability and membrane potential.

Rapid BDNF Upregulation

Researching the signaling pathways through which PE-22-28 triggers a fast-acting increase in BDNF levels compared to traditional research models.

Hippocampal Neurogenesis

Studying the peptide’s influence on the proliferation and maturation of new neurons within the dentate gyrus.

Synaptic Plasticity Models

Exploring the role of PE-22-28 in enhancing excitatory synaptic transmission and the structural remodeling of dendritic spines.

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Technical Specifications

Parameter	Laboratory Standard
Compound	PE-22-28 (Spadin Analog)
Sequence	Gly-Val-Trp-Arg-Asn-Gly-Arg
Purity (HPLC)	≥99% (Pharmaceutical Grade)
Physical Appearance	Fine, Smooth & Flat Lyophilized Cake
Molecular Weight	829.9 Da
Concentration	Available in 5mg / 10mg vials
Solubility	Highly Soluble in Water (Aqueous) / Bacteriostatic Water
Batch Control	MS & HPLC Analysis Verified

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Key Features & Quality Assurance

Pharmaceutical-Grade Lyophilization

Our proprietary vacuum-sealing process ensures the PE-22-28 powder surface is perfectly smooth, flat, and delicate. This uniform “cake” structure ensures rapid reconstitution and protects the heptapeptide sequence from oxidative degradation during long-term storage.

Enhanced Molecular Stability

Compared to the original Spadin sequence, our synthetic PE-22-28 is optimized for improved stability in research media, allowing for consistent data collection in longitudinal in-vitro studies.

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Reconstitution & Storage

Reconstitution Guide

• Preparation: Allow the vial to reach room temperature. Centrifuge for 30-60 seconds to ensure the PE-22-28 cake is at the base.

• Solvent: Reconstitute with Sterile Water or Bacteriostatic Water.

• Technique: Gently introduce the diluent against the vial wall. DO NOT SHAKE; swirl gently until the solution is clear and transparent.

Storage Protocols

• Long-term: Store at -20°C (Maintains potency for up to 24 months).

• Short-term: Stable at 2–8°C for up to 3 months.

• Post-Reconstitution: Store at 2–8°C and utilize within 10-14 days for optimal results.

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Shipping & Documentation

• Global Logistics: Shipped in discreet, temperature-controlled packaging to maintain the structural integrity of the PE-22-28 peptide.

• Verified Quality: A batch-specific Certificate of Analysis (COA) and HPLC/MS reports are available for every order.

• Reshipment Policy: We provide a guaranteed reshipment policy for any lost or customs-intercepted orders (terms apply).

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⚠️ Research Disclaimer

Product-Specific Safety Notice: This PE-22-28 is strictly for laboratory research and analytical purposes. It is NOT intended for human or veterinary administration. All research must be conducted by qualified professionals.

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Scientific Resources & References

• National Center for Biotechnology Information (NCBI): Compound Summary – PE-22-28 (spadin-derived peptide; sortilin-related fragment)

• PubMed – NIH: Research on PE-22-28 / spadin peptides targeting TREK-1 potassium channels and antidepressant effects

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🔍 Deep Dive Research

• [Technical Guide]: How to Reconstitute and Store Lyophilized Peptides for Maximum Potency

• [Neuroscience Study]: Beyond Monoamines: The Role of PE-22-28 in Fast-Acting Neurogenesis Research