Description

PNC-27 Peptide

PNC-27 is a membrane-active synthetic peptide derived from the p53 tumor suppressor protein (specifically residues 12-26) attached to a membrane-resident carrier peptide. In the field of molecular oncology and cellular biology research, PNC-27 is highly distinguished by its unique ability to selectively target the HDM2 (Human Double Minute 2) protein expressed on the cell membranes of various cancer cell lines. Unlike many traditional research compounds, PNC-27’s mechanism involves inducing rapid membrane lysis (pore formation), leading to the necrosis of malignant cells while sparing healthy cells that do not express membrane-bound HDM2. This provides researchers with a high-precision model to study p53-independent cell death and membrane-targeted therapeutic strategies.

Our PNC-27 is synthesized in an ISO 9001:2015 certified and GMP-grade manufacturing environment. Utilizing advanced Solid-Phase Peptide Synthesis (SPPS) and rigorous multi-stage HPLC purification, we achieve a chemical purity of ≥99%. This meticulous production standard ensures the peptide is free from truncated fragments and residual synthesis reagents, guaranteeing the consistency required for high-stakes studies into membrane poration and selective cytotoxicity.

---

Research Applications & Mechanisms

HDM2-Mediated Membrane Targeting

Investigating the peptide’s specific binding affinity for HDM2 proteins expressed on the plasma membranes of malignant versus non-malignant cell models.

Mechanisms of Membrane Lycolysis

Researching the rapid pore-formation (necrosis) induced by PNC-27 and its influence on cellular osmosis and structural integrity.

p53-Independent Cell Death Pathways

Studying how PNC-27 induces tumor cell necrosis without requiring the activation of the endogenous p53 pathway or apoptotic signaling.

Broad-Spectrum Oncology Models

Exploring the efficacy of PNC-27 in various research environments, including solid tumor models and hematological malignancy studies.

---

Technical Specifications

Parameter	Laboratory Standard
Compound	PNC-27 (p53 12-26 Fragment Blend)
Purity (HPLC)	≥99% (Pharmaceutical Grade)
Physical Appearance	Fine, Smooth & Flat Lyophilized Cake
Molecular Weight	4431.2 Da
Concentration	5mg per vial
Solubility	Highly Soluble in Water (Aqueous) / Bacteriostatic Water
Batch Control	MS & HPLC Analysis Verified

---

Key Features & Quality Assurance

Pharmaceutical-Grade Lyophilization

Our proprietary vacuum-sealing process ensures the PNC-27 powder surface is perfectly smooth, flat, and delicate. This uniform “cake” structure ensures rapid reconstitution and maintains the structural integrity of the complex peptide chain during storage.

Sequence Integrity & Fidelity

Every batch undergoes strict Mass Spectrometry (MS) to confirm the sequence identity and HPLC to verify purity levels exceed research requirements, ensuring reproducible data in cellular assays.

---

Reconstitution & Storage

Reconstitution Guide

• Preparation: Allow the vial to reach room temperature. Centrifuge for 30-60 seconds to ensure the PNC-27 cake is at the base.

• Solvent: Reconstitute with Sterile Water or Bacteriostatic Water.

• Technique: Gently introduce the diluent against the vial wall. DO NOT SHAKE; swirl gently until the solution is clear and transparent.

Storage Protocols

• Long-term: Store at -20°C (Maintains potency for up to 24 months).

• Short-term: Stable at 2–8°C for up to 3 months.

• Post-Reconstitution: Store at 2–8°C and utilize within 7-10 days for optimal results.

---

Shipping & Documentation

• Global Logistics: Shipped in discreet, temperature-controlled packaging to maintain the structural integrity of the PNC-27 peptide.

• Verified Quality: A batch-specific Certificate of Analysis (COA) and HPLC/MS reports are available for every order.

• Reshipment Policy: We provide a guaranteed reshipment policy for any lost or customs-intercepted orders (terms apply).

---

⚠️ Research Disclaimer

Product-Specific Safety Notice: This PNC-27 is strictly for laboratory research and analytical purposes. It is NOT intended for human or veterinary administration. All research must be conducted by qualified professionals in controlled environments.

---

Scientific Resources & References

• National Center for Biotechnology Information (NCBI): Compound Summary – PNC-27 (p53-derived anticancer peptide)

• PubMed – NIH: Research on PNC-27 anticancer peptide and tumor cell membrane interaction

---

🔍 Deep Dive Research

• [Technical Guide]: How to Reconstitute and Store Lyophilized Peptides for Maximum Potency

• [Oncology Study]: Selective Cytotoxicity: Understanding the PNC-27 Membrane-Targeting Mechanism