Description

TBF (Thymosin Beta-4 Fragment / TB-500)

TBF, commonly known in laboratory circles as TB-500, is a synthetic, acetylated 20-amino acid fragment of the naturally occurring protein Thymosin Beta-4. Unlike the full-length protein, this specific fragment contains the essential G-actin binding domain, which is responsible for its high biological activity in tissue regeneration and cellular migration. In biochemical research, TB-500 is a primary focus for investigating the mechanisms of wound healing, muscle recovery, and the stimulation of angiogenesis (the formation of new blood vessels) within injured tissue microenvironments.

Our TB-500 is synthesized in an ISO 9001:2015 certified and GMP-grade manufacturing environment. By utilizing advanced Solid-Phase Peptide Synthesis (SPPS) and multi-stage HPLC purification, we guarantee a chemical purity of ≥99%. This meticulous quality control ensures that the peptide is free from truncated fragments and residual synthesis reagents, providing the high-fidelity results necessary for sensitive research into cellular signaling and extracellular matrix remodeling.

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Research Applications & Mechanisms

Cellular Migration & Wound Healing

Investigating the peptide’s ability to upregulate actin, thereby promoting the migration of cells to the site of injury for accelerated tissue repair research.

Angiogenesis & Vascular Research

Studying the pathways through which Tβ4 fragments stimulate endothelial cell differentiation and the development of new capillary networks.

Anti-Inflammatory Signaling

Researching the modulation of pro-inflammatory cytokines and the peptide’s role in reducing oxidative stress within damaged muscular and connective tissues.

Cardioprotective Models

Exploring the regenerative effects of TB-500 in cardiac tissue models to observe its influence on cellular survival and structural stability.

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Technical Specifications

Parameter	Laboratory Standard
Compound	TBF (Thymosin Beta-4 Fragment / TB-500)
Production Quality	GMP-Level Compliance
Purity (HPLC)	≥99% (Pharmaceutical Grade)
Physical Appearance	Fine, Smooth & Flat Lyophilized Cake
Molecular Formula	C103H162N26O32S
Molecular Weight	2236.6 Da
Solubility	Highly Soluble in Water (Aqueous) / Sterile Saline
Batch Control	MS & HPLC Analysis Verified

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Key Features & Quality Assurance

Pharmaceutical-Grade Lyophilization

Our proprietary vacuum-sealing process ensures the TB-500 powder surface is perfectly smooth, flat, and delicate. This uniform “cake” structure ensures rapid reconstitution and protects the 20-amino acid chain from environmental degradation.

Acetylated Stability

The synthetic acetylation of this fragment enhances its stability in research media, allowing for consistent observations in both in-vitro and in-vivo experimental designs.

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Reconstitution & Storage

Reconstitution Guide

• Preparation: Allow the vial to reach room temperature. Centrifuge for 30-60 seconds to ensure the TBF cake is at the base.

• Solvent: Reconstitute with Sterile Water or Sterile Saline (0.9% NaCl).

• Technique: Gently introduce the diluent against the vial wall. DO NOT SHAKE; swirl gently until the solution is clear and transparent.

Storage Protocols

• Long-term: Store at -20°C (Maintains potency for up to 24 months).

• Short-term: Stable at 2–8°C for up to 3 months.

• Post-Reconstitution: Store at 2–8°C and utilize within 10-14 days.

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Shipping & Documentation

• Global Logistics: Shipped in discreet, temperature-controlled packaging to protect the TB-500 molecular structure.

• Verified Quality: A batch-specific Certificate of Analysis (COA) and HPLC/MS reports are available for every order.

• Reshipment Policy: We provide a guaranteed reshipment policy for any lost or customs-intercepted orders (terms apply).

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⚠️ Research Disclaimer

Product-Specific Safety Notice: This TBF (Thymosin Beta-4 Fragment) is strictly for laboratory research and analytical purposes. It is NOT intended for human or veterinary administration. All research must be conducted by qualified professionals.

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Scientific Resources & References

• National Center for Biotechnology Information (NCBI): Thymosin Beta-4 Related Compounds (CID 16132341)

• ScienceDirect (Elsevier): Research on Actin-Binding Peptides and Tissue Regeneration

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🔍 Deep Dive Research

• [Technical Guide]: How to Reconstitute and Store Lyophilized Peptides for Maximum Potency

• [Regenerative Study]: The Role of TB-500 in Modern Angiogenesis and Wound Repair Research